Sojasauce (Tamari, bio? Roh?)

Weisse Sojasauce-Shiro
Weisse Sojasauce ist nicht wirklich weiss, sondern honigfarben. Sie hat einen sehr hohen Weizenanteil und einen geirngen Sojabohnenanteilm die Fermentaionszeit ist kurz. Besonders gut für klare Brohen und Eiergerichte. Man kann damit abeer auch westliche Consimmé den letzen Schliff verleihen

According to connoisseurs, the shoyu bouquet has more than two hundred constituent, which result from the lenghty fermentation process. For the sauce to acquire this complexity takes 12 to 24 months by traditional production methos. The basic ingredients used are soybeans, wheat, spring water, koji, and seas salt. The wheat is first roasted and cracked. The soybeans are soaked in water and steamed. Both are then mixed together, an the whole is inoculated ith koji mold, a fermentation starter. Ober the next 40 hours, the koji multiply in the mixture. The sweet-smelling brew is then combined with salted spring water and left to feremnt in a wooden barrel for up to one year. While the high salt content prevents unwanted bacteria from growing in the barrel, proteins are broken down into amino acids, and starch into sugars, alcohol, and organic acids. These give shoyu its rich flavor and hundreds of aromatic compounds. Fermentation also increases the nutritional benefits uf shoyu. The bacteria synthezize enzymes and vitamins, including vitamnin B12 and create a more usable amino-acid balance. After fermentation and aging, the mixture is pressed to seperate the liquid, shoyu, from the mash. The shoyu is filtered and then heat--treated, to stop the fermentation, sterilize the shoyu, and add an extra flavor component-a sort of caramelized flavor. Today advanced refrigeration and transportation technologies in Japan allow the producers to distribute non-heat-treated shoyu, called kijoyu or namashoyu, which is still biologically active. Unpasteurized shoyu has a distinctive robust, natural flavor that some people prefer. Much of the shoyu made by large manufacturers is produced in a shortened, more automated way. Fermentation is done in termerture-sontrolled tanks, so production time can be reduced to only four to five months. This results in shoyu whith less flavor than traditionally made soy sauce.

A japanese kithen uses three types of shoyu, which differ in color, flavor and degree of saltiness. the most widely available, both in Japan and overseas, is koikuchi shoyu. This type is also called simply shoyu, the name I use in this book. Koichuchi shoyu has a dark brown color, rich flavor and compley aroma. The salt content of koikuchi shoyu is 17 to 18%; that is , 1 tabespoonn contain over 1/2 teaspoon salt. Koikuchi shoyu is used with all types of ingridients- poultry, meat, fish, and vegetables- and in all types of preperations-sauces, soups, and braised and stir-fried dishes. Koichuchi shoyu gives food a dark brown and rich flavor. 
The second type of Japanese soy sauce is usukuchi shoyu, "light-colored soy sauce ". To produce light colored shoyu, the wheat is more lightly roasted, and more salt is added to slow the fermentation. A small amount of mirin, sweet rice wine, may also be added. The resulting shoyu is lighter in color, less strongly flavored, and slightly higher in salt content thant regular shoyu, about 19 %. Because of these charcteristic, usukuchi shoyu is used in recipes where a refined color and weak flavor are required.
The third kind of soy sauce, tamari, is traditonally produced in only three Japanes prefectures. Aichi, Gifu and Mie. Tamari is made nearly entirely from soybeands, and only a very small amount of water is adde to the fermentating mixture. After 20 month of fermentation, tamari is thicker than the other types, dark brown in color, rich in bean flavor, and about as salty as regular shoyu. Unlike the two other types of shoyu, tamari is prefered as a condiment of flavor enhancer rather than as a basic cooking ingridient. Tamari may also be added to dishes at the end of the cooking process. 

While the miso is fermenting in the tub, juices leak from the bottom and are collected underneath. This juice is calles tamari or taremiso-used to make soy sauce. In the US, there is a product named "tamari". This is completely different from the orifinal tamari collected from the fermentating miso. If you have experienced Japan, you know that American futons compared to Japanese futons: the name are the same, but the two are fundamentally different.

tamari ... By-product from making miso used as a flavoring

Soy sauce made from soy (tamari).

1tbsp 18 g

1tsp 6 g

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The antioxidative potency of soy sauce is often linked to polyphenols, melanoidins, free amino acids and low molecular weight peptides, which play roles in diminishing oxidative damage by clearing free radicals and reactive oxygen species.

The phenolic molecules identified in soy sauce are classified as simple isoflavones, furanones, pyranones and phenolic acids. In particular, the presence of 4-ethylguaiacol, catechol, daidzein and 4-ethylphenol is responsible for the antioxidative activity of soy sauce. 

The products of the Maillard reaction produce the antioxidant characteristics in soy sauce. Among them, melanoidins were the major substances of brown color products in soy sauce and showed strong antioxidant activities (Wang et al., 2007).

Amino acids and small peptides are the main fractions of total nitrogen in soy sauce, and both accounted for 45% of the total nitrogen. A total of 31 free amino acids were detected in the soy sauce sample. The predominant free amino acids in the fermented soy sauce were proline (8.4%), leucine (7.78%) and aspartic acid (7.13%), in descending order (Lin et al., 2016). Free amino acids had effective ABTS and DPPH radical-scavenging activities and metal-ion chelating capacities. 

Shoyu effectively inhibited ACE activity, thereby regulating the blood pressure in SHRs and in Dahl salt–sensitive rats (Nakahara et al., 2010). Several ACE inhibitory peptides, including Ala-Trp, Gly-Trp, Ala-Tyr, Ser-Tyr, Gly-Tyr, Ala-Phe, Val-Pro, Ala-Ile, and Val-Gly, were identified in shoyu, and nicotianamine, an ACE inhibitory compound, was also isolated from shoyu (Nakahara et al., 2010).

Anti-inflammatory and immunomodulatory components

The immune regulation and anti-inflammation of soy sauce polysaccharides (SPS) have been reported in multiple studies (Table 1). 

4.5. Anticancer components: Soy sauce is also reported to have anticancer effects.

Narrativer Review in wissenschaftlichem Journal 

DOI: 10.1016/j.foodres.2022.111575

Study: weak evidence

Soy sauce is consumed not only because of its color and flavor but also because of  its health benefits. Soy sauce exhibits antioxidant (Hsiao et al., 2013),  antihyperuricemic (Li et al., 2017), antimicrobial, anticarcinogenic, anticataract, and  antiplatelet activities (Kataoka, 2005). This product contains many antioxidant  substances, including soy isoflavones, melanoidins, furan ketones, phenolic acids, organic acids, peptides, and β-carbolines (Li et al., 2017).

Experimentelle Studie in wissenschaftlichem Journal

DOI: 10.1016/j.lwt.2018.01.008

Study: weak evidence

It is well established that high levels of biogenic amines in foods constitute a potential public health concern due to toxicological effects. Histamine at levels higher than 100 mg/kg, tyramine levels higher than 100 mg/kg, and phenylethylamine levels above 30 mg/kg can cause adverse effects to human health (Gloria, 2005; Rauscher-Gabernig et al., 2009; Yongmei et al., 2009). Based on this criterion, 48% of the samples could cause histamine poisoning; 61% could induce migraine headache due to tyramine; and 31% could cause headache due to phenylethylamine (Table 5).

Experimentelle Laborstudie in wissenschaftlichem Journal

DOI: 10.1016/j.foodchem.2012.01.033

Study: weak evidence

fermented products (miso, tamari, soy sauce, natto, tempeh, soybean paste and sufu), were purchased from Spanish local stores. Three batches from one brand for each type of product were studied and examined by duplicate for biogenic amine and polyamine contents.

On the contrary, miso, tamari, soy sauce, natto, tempeh, soybean paste and sufu are fermented products, most of them imported from Asian countries. So, the specific conditions used for their fermentation and manufacturing processes are unknown. With the exception of soy sauce, the other products are scarcely distributed in the Spanish market, mainly found in specific shops. Samples were kept at 30 C until their analyses.

The physiological polyamines spermidine (SPD) and spermine (SPM) are present in all animal and vegetal tissues and their origin is not related to microbial activity. On the contrary, the called biogenic amines, mainly including tyramine (TY), histamine (HI), tryptamine (TR), b-phenylethylamine (PHE), putrescine (PU) and cadaverine (CA), are present in food as a consequence of bacterial enzymatic decarboxylation of their precursor amino acids. In spite of their recognised bacterial origin, PU and CA could be also considered as naturally occurring amines in some foods since they can be synthesized at low amounts in both vegetal and animal organisms.

Adverse effects of biogenic amines as a result of consumption of tofu, soymilk, soybean sprouts, miso, tamari, soy sauce, natto, tempeh, soybean paste and sufu would be unlikely in healthy population, but some of them, especially sufu and soybean paste, should be taken into account in individuals taking MAOIs and DAOIs drugs. On the other hand, some fermented soybean products, such as tempeh and natto, showed to be an important source of polyamines, which appears to have a positive effect on health, except for patients suffering from cancer.

Laborstudie in wissenschaftlichem Journal

DOI: 10.1016/j.foodchem.2014.10.118

Study: weak evidence

Although soybean is a rich source of plant protein, its nutritive values are concealed by the anti-nutritive properties. Fermentation not only reduces the toxins but it leads to the production of several beneficial compounds such as peptides. Peptides in fermented soybean products exhibit bioactive properties such as ACEinhibitory, antioxidant, antitumor, antidiabetic and antimicrobial property. The bioactive peptides produced during fermentation are produced either by starter culture or by hydrolysis of soybean proteins. Studies on the bioactive peptides in fermented soybean products responsible for such properties will result in their application in preparation of functional food and food supplements. Also, specific starter cultures are responsible for the production of specific peptides, and the same is applicable with the specific strains of culture. From the studies till date, it can be concluded that peptides are one of the promising health promoting components in fermented soybeans products. Future studies on other health benefits and characterization of bioactive peptides in fermented soybean can lead to its application as pharmaceuticals with specific health benefits and functional foods to fight against degenerative diseases to replace the synthetic drugs which have surplus side effects.

Narrativer Review in wissenschaftlichem Journal

DOI: 10.1016/j.tifs.2016.01.010

Study: weak evidence

Recommendations for salt reduction

• For adults, WHO recommends less than 2000 mg/day of sodium (equivalent to less than 5 g/day salt (just under a teaspoon).
• For children aged 2–15 years, WHO recommends adjusting the adult dose downward based on their energy requirements. This recommendation for children does not address the period of exclusive breastfeeding (0–6 months) or complementary feeding with continued breastfeeding (6–24 months).
• All salt that is consumed should be iodized (fortified with iodine), which is essential for healthy brain development in the foetus and young child and optimizing people’s mental function in general.

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Soy sauce is a fermented product, and its flavor is a complex mixture of individual senses which, in combination, create a strong palatable condiment for many Eastern and Western dishes. This Review focuses on our existing knowledge of the chemical compounds present in soy sauce and their potential relevance to the flavor profile. Taste is dominated by umami and salty sensations. Free amino acids, nucleotides, and small peptides are among the most important taste-active compounds. Aroma is characterized by caramel-like, floral, smoky, malty, and cooked potato-like odors. Aroma-active volatiles are chemically diverse including acids, alcohols, aldehydes, esters, furanones, pyrazines, and S-compounds. The origin of all compounds relates to both the raw ingredients and starter cultures used as well as the parameters applied during production. We are only just starting to help develop innovative studies where we can combine different analytical platforms and chemometric analysis to link flavor attributes to chemical composition.

Narrativer Review in wissenschaftlichem Journal

DOI: 10.1021/acs.jafc.0c04274

Study: weak evidence

Melanoidins, generated during the Maillard reaction in soy sauce fermentation, have potential health benefits due to its excellent bioactivity. This study aimed to investigate the antioxidant contributions and structural characteristics of melanoidins in soy sauce. Five molecular weight fractions (1–3 kDa, 3–10 kDa, 10–30 kDa, 30–50 kDa, and >50 kDa) were isolated and their composition was analyzed. Results showed that soy sauce melanoidins mainly comprised proteins, sugars, and phenolic compounds. Antioxidant activities of the melanoidins were influenced by their molecular weights and structures. The >50 kDa melanoidins fraction contributed the most to the overall antioxidant activities of soy sauce. The total contributions of melanoidins to the antioxidant activities of soy sauce ranged from 34.21% to 75.03%. Spectroscopic analyses indicated that the antioxidant activities were positively correlated with the presence of conjugated structures and active functional groups (i.e., C=C, C=O, N-H, O-H) in melanoidins. This study provides new insights into the health-promoting properties of soy sauce melanoidins and offers theoretical support for the development of soy sauce as a functional food.

Experimentelle Studie in wissenschaftlichem Journal

DOI: 10.3390/foods14162787

Study: weak evidence

At the beginning of the twentieth century, the Japanese chemist Kikunae Ikeda identified the glutamate ion as inducing a novel taste quality that he proposed was a signal for protein, analogous to sweetnes being a signal fo energy or calories. He described this novel quality as "the peculiar taste we feel as umami (meaning, according to the translator, meaty, brothy, or savory)". He calle sthis taste umami. Subsequent studies further identified the ribonucleotides 5'-inosinate and 5'-guanylates as synergistic enhancers of this novel taste, altough for humans and some other species, they may not produce a taste on their own. Beginning in the late 1960s and 70s, additional researchers also proposed umami as a novel fifth basic taste. This idea gained momentum from a scientif meeting held in 1985 and the publication of the proceedings Umami: A Basic Taste. The evidence used to support the proposal that umami was the fifth basic taste at the time sonsisted primarly of human sensory studies and animal model studies of behavior and physiology.
The discory in the 1990s and 2000s of specific taste receptors responsive to amino acids gave substanial impetus to the idea that umami might be the fifth basic taste. Yet even the proponents of umami as basic taste acknowledge that it differs significally from classic fou : it is more subtle, which may account for it no being identified historically or in most traditional cultures, and unlike the other appetitive taste qualities, swwt and salty, relatively pure solutions of monosodium glutamate are very rare in natrue and are not palatable for hman adults or infants. Moreover, unlike sweet, salty and bitter, the perceptual quality of umami for humans does not directly signal the presence of purporte nutrient, proteins (which gernally have no taste), or even amino acis. Indeed, many umami-rich foods are not naturally high in protein.

..it signals easily digested protein as formed during cooking and fermentation. He noted thta fermented foods also have the nutrional advantage of providing easy acess not only to amino acids but also to probiotic bacteria

Book: moderate evidence

Tamari's history dates back to 7th century AD, when it was brought to Japan from China. It has been known since ancient times that cooked and fermented soybeans will produce a tasty, dark red paste, which the Japanese call miso. During the miso ripening process, a protein-rich liquid accumulates. This liquid was given the name tamari, or "that which accumulates." Tamari is the origin of Japanese soy sauce.

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THE ROOTS OF SOY SAUCE ARE IN CHINA
It’s said that the roots of soy sauce can be traced back to a sauce called “jan” in ancient China. That began from pickling raw materials in salt to preserve them, and there were varieties based on fruit, vegetables, and seaweed etc., on meat and fish, on meat only, and on grains. The grain type, using rice, wheat, and soybeans, is thought to be the archetype of soy sauce. It is not clear when it came to Japan, under the name “hishio”, but according to the Taiho Code, “hishio” made from soybeans was to be made at the hishio institute belonging to the cuisine division of the Imperial Household Agency. In modern terms, that hishio was midway between soy sauce and miso paste, and appears to have reached the dining tables of palace banquets. After that, the making of miso paste was begun using the Kinzanji method that the Zen monk Kakushin brought back from China in 1254 (in the Kamakura era). It is said that while he was teaching that miso-making method to the villagers of Kishu Yuasa, he noticed that the liquid that seeps out of hishio tasted really good, and that became what is now known as “tamari soy sauce”.

JAPANESE SOY SAUCE WAS BORN IN THE KANSAI REGION
From volume 5 of “Koeki Kokusanko”, an agricultural chronicle of the Edo period, on soy sauce manufacturing
The soy sauce manufacturing methods that is said to have been born in Kishu Yuasa kept on advancing after that. Around 1580 (Tensho era) , Tamai soy sauce, thought to be the first soy sauce vendor in Japan, started selling soy sauce and miso paste. There is a record from 1588 (Tensho year 16) of approximately 18,000L of tamari soy sauce being sent from Kishu to Osaka. This suggests that soy sauce was already a daily necessity among the townspeople of Osaka. In Kanto, on the other hand, soy sauce had arrived but was not being manufactured. It was being brought from Kansai. It was called “Kudari” soy sauce, which means it had been brought from the capital.

The internationalization of Japanese soy sauce actually began in the Edo era. Japan was then in its period of national isolation, but trade was permitted between Nagasaki and Holland as the only exception. The Dutch ships and Chinese ships started carrying Japanese soy sauce to the Chinese mainland, other parts of Southeast Asia, and as far as Holland. The soy sauce exported in that period was local soy sauce produced in Sakai in Osaka, in Kyoto, and in Kyushu. Other than shipping in barrels, it appears to have been sent in earthenware bottles called “konpura bottles”. After that, soy sauce was loved around the world as a versatile condiment. It’s popularity joined with the current boom in Japanese food to put soy sauce on meal tables around the world.

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The brewing of traditional soy sauce involves a two-stage primary fermentation process, a short-term solid-state fermentation (koji) followed by long-term liquid-state fermentation (moromi) (Yee et al., 2021). The microorganisms in the koji fermentation step, mainly members of the Aspergillus genus (A. oryzae or A. sojae), produce enzymes that breakdown soybean and wheat into simple molecules used for the development of halophilic bacteria including lactic acid bacteria (LAB), Bacillus species, and yeasts during the moromi stage with 18%–20% salt content. The mash is allowed to ferment for 1 month to 4 years of maturation to finally produce soy sauce (Det-udom, Gilbert, et al., 2019; Harada et al., 2017). Apart from fermentation, soy sauce can also be made via chemical hydrolyzation by adding hydrochloric acid to hydrolyze the soybean protein to shorten the production time from several months to 1–3 days. Although this method is cheaper and faster, the soy sauce quality is unsatisfactory because of the insufficient aromatic and flavor substances as well as the presence of some undesirable compounds that are lacking in naturally fermented soy sauce, including carcinogens such as 3-chloropropane-1,2-diol (3-MCPD) and 1,3-dichloropropanol (1,3- DCP; Lee et al., 2006; Liu, 2012; Luh, 1995). Despite the favorable image of soy sauce in terms of nutrition and health benefits as well as its recent production progress, several limitations need to be resolved. For instance, numerous attempts have been made to reduce the considerable fermentation time during the moromi stage using genetic engineering approaches as well as cell and enzyme immobilization without altering the quality of the final product (Devanthi & Gkatzionis, 2019). Furthermore, the high salt content in soy sauce is a cause of concern due to health impacts such as hypertension and renal dysfunction. Thus, driven by industry and the World Health Organization recommendation to reduce the dietary intake of sodium salt, the amount in soy sauce has been reduced to ~8%–11%. However, salt is a critical element in the fermentation of soy sauce because it regulates microbial and organoleptic properties; thus, its reduction will not only cause microbiological safety concerns but could also compromise the final product quality (Devanthi, Linforth, El Kadri, et al., 2018; Devanthi, Linforth, Onyeaka, et al., 2018; Hoang et al., 2016). Another concern is the by-products generated during manufacturing, including ethyl carbamate (EC), a Group 2A carcinogen formed by arginine-consuming bacteria in the moromi mash. 

Narrativer Review in wissenschaftlichem Journal

DOI: 10.1111/jfpp.15799

Study: weak evidence

Soy sauce, commonly called shoyu, is made from soy bean, wheat and salt.9 Soy bean is the primary ingredient. Other ingredients and additives of soy sauce are salt, alcohol, amino acid, glucose and sugar. Shoyu is made by brewing soybean; there were some brewing process stages, koji, moromi and raw soy sauce.9 Soybean change koji at first stage, then koji change moromi. At last stage, moromi change raw soy sauce. Beneficial effects of soy sauce are hypolipidaemia,13 improvement of anaemia,14 promotive secretion of gastric juice,15 anti-allergic, inhibitory allergic reactions16 etc., whereas it is well-known that some people suffered irritation or dermatitis or cellulites after using shoyu in the past. It is possible that the process of brewing soy sauce is what generates the adverse effects: soy sauce allergy, histamine poisoning, soy allergy, wheat allergy, etc. Soy bean and wheat are major allergens of food allergy; major allergens of soybean are Gly mBD 30k8 and Gly mBD 28k17 which were measured using ELISA.8 Soy sauce and fermented foods are considered hypoallergenic foods and any allergens were not detected1 because these allergens degrade during fermentation.1,18 Shoyu-flavones are produced during fermentation and shoyu-flavones inhibit histidine decarboxylase, which results in the production of histamine.19 Histamine is made during the brewing process of soy sauce.12,20 We noted allergy of soy sauce and histamine poisoning and investigated the volume of histamine in some kinds of soy sauce. The results were interesting in that histamines were detected in the sauces made from soybeans and broad beans,but not in the sauces made from millet (awa), barnyard grass and fish. The colours of sauces that contained histamine were deep. We suspected that one cause of the patient symptoms was histamine poisoning and then we studied it with prick test using soy sauce with nine volunteers. If histamine poisoning causes cellulites or dermatitis around the lip, the results of prick test to all volunteers must be positive. In fact, volunteers had negative reactions by prick test after using soy sauce as positive reactions to soy sauce in four patients were not caused by histamine poisoning. These four patients did not develop any histamine poisonings after eating histamine-contained foods11,12 or histamine-releasing foods (additives, citrus fruit, strawberries, chocolate, tomatoes, peanut, etc.)12 or some ingredients and additives of soy sauce (salt, alcohol, amino acid, glucose and sugar). These four patients did not have any allergy of ingredients or additives in sauce from the results of prick test or after eating meal; they had allergy of some products in sauce during brewing. At this time, although patients were female, the relationship between gender and soy sauce allergy is not definite. It is speculated that soy sauce allergy is caused by substances raised during fermentation. It was difficult to detect what allergen, a generative product of soy sauce, causes their allergic reactions. As soy sauce is consumed globally, the number of patients having soy sauce allergy or histamine poisoning of soy sauce could increase. The manufactures and companies of soy sauce should educate or acquaint with people not only about the advantageous effects but also about histamine poisoning or allergic reactions.

Fallserie

DOI: 10.1111/j.1468-3083.2009.03512.x

Study: weak evidence

The Japanese have declared it their National Mold and have even created comic books in which it is featured as a catoon caracter.
Koji mold, Aspergillus oryzae (or, as we will refer to it going foward, simply koji), has a bit of mystique surrounding its origin. Due to rigorous scientific research, we know that koji envolved when it was domesticated from the highly toxic A. flavus. Dr. John Gibbons at Clark University in Worcester, Massachusetts, is currently leading the way in research related to how koji evolved away from its toxic ancestor, by identyfying the traits and genetic changes that accompanied the domestication of A. oryzae. To adress this topic he and his team sequence and compare the genomes of A. oryzae and its toxic progenitor species, A. flavus. They then use computational genomics, ecolutionary biology, and population genetics to pinpoint genetic differences between domesticated and wild genomes. When the geenetic differences between domesticated and wild genomes. When the genetic ..
It confirmed that humans have been making rice-based alcohol for nine thousand years through the help of filamentous fungi. 
For more than ten thousand years humans have been taming plants and animals for particular charactteristics. For example, domesticated plants usually produce more fruits or seeds than their wild progenitors thanks to selective breeding. Domestication has a profound impact on the genome of any given organism, and koji is no exception. 

Book: moderate evidence

The results showed that it was possible to achieve a NaCl reduction in the tested foods of, respectively, 50%, 17%, and 29% without leading to significant losses in either overall taste intensity or product pleasantness. These results suggest that it is possible to replace NaCl in foods with naturally brewed soy sauce without lowering the overall taste intensity and to reduce the total NaCl content in these foods without decreasing their consumer acceptance.

Practical application: Health concerns related to salt consumption cause an increased demand for salt-reduced foods. Consequently, the development of foods with reduced salt content without decreasing the consumer acceptance is an important challenge for the food industry. A new possible salt reduction approach is described in the present article: The replacement of salt with naturally brewed soy sauce.

Experimentelle Interventionsstudie ohne Randomisierung

DOI: 10.1111/j.1750-3841.2009.01232.x

Study: moderate evidence

The average water footprint for 330 ml of soy sauce was 200 litter. 

The scope of the study only focuses on one-unit stock of a particular brand located in Kuala Kangsar, Perak in Malaysia. 

Ökologische Ressourcenanalyse

DOI: 10.26480/jwbm.01.2020.01.04

soy sauce glass bottle:Live footprint: 1.2 kg CO₂e/kg

Live footprint: 0.65 kg CO₂e/kg (Shoyu mit Weizen und Soja aus NL)

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The total water footprint of soy sauce production is 1.986,35 L/kg with fraction of green water (78,43%), blue water (21,4%) and gray water (0,17%). 

The raw material of soy sauce are soybean, brown sugar and salt. There are 3 main stage in the process of soy sauce production is boiling and drying soybean, fermentation process and mixing and cooking soy sauce.  

Ökologische Ressourcenanalyse

DOI: 10.1051/e3sconf/20183108018

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mustard, Ketchup, table salt

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